Alamo®  Labs   210-308-0427
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Custom Gene Synthesis

Custom Gene synthesis and Cloning

Cat No Custom Service Description
MBS-01

Custom Gene synthesis

MBS-02 Constructs for Adenovirus Expression System
MBS-03 Constructs for Baculovirus Expression System
MBS-04 Construction of Gene-specific SiRNA
MBS-05 Sub-cloning
MBS-06 PCR Cloning
MBS-07 RT-PCR
MBS-08 cDNA library construction









 

Alamo® Labs offers state of the art gene synthesis service with no set up fee. We try our best to achieve synthesis of difficult genes with sequence repeats or GC rich contents. We codon-optimize gene for expression in organism of your choice as well as sub-cloned it into pUC or pBSK vector with no extra charge. We can sub-clone your gene into vector of your choice for a nominal fee. You'll have to provide vector of your choice. For a quick non-binding quote please e-mail gene sequence to Service@alamolabs.com .

Cloning & Sub-cloning Service

Constructs for Adenovirus Expression System

It is a very strong and efficient system for the simultaneous expression of two or more specific gene(s) in mammalian cells. We provide this service for the construction of your gene of interest. Toxin-free final product is ready to transfect HEK cells for the production of recombinant adenovirus.

Constructs for Baculovirus Expression System

One of the most widely used gene(s) expression system for production of recombinant proteins in Eukaryotic cells (such as sf9, Sf21). We offer cloning services for the construction of gene of your interest in baculovirus-based expression vector(s). Final product is ready to transfect insect cells for the packaging of recombinant baculovirus.

Construction of Gene-specific SiRNA

This revolutionary technique to block the expression of a specific protein(s) in cells or animals has proven to be a reliable and quick approach. We'll take care of your SiRNA construct from designing of the specific primers to its cloning in an SiRNA expression vector. We also provide SiRNA duplex synthesis service with desired modifications such as biotin.

Sub-cloning

This service is provided for sub-cloning of gene/cDNA into vector of your choice. For accuracy, recombinant clones will be verified by restriction analysis. A project report with detail analysis and 10 ug of final product will be supplied. Customer supplies the specific gene/ cDNA and vector.

PCR Cloning

We'll design and make gene-specific primers for PCR amplification from a cDNA library. We'll buy commercially available specific cDNA library if customer can not provide it. PCR amplified cDNA fragment will be sub-cloned in TA cloning vector or in a vector of your choice. Recombinant clones will be verified by restriction fragment analysis. For accuracy, two selected clones will be sequenced. A project report with detail analysis and 10 ug of final product will be supplied.

RT-PCR

We'll reverse transcribe and PCR amplify specific gene from customer’s provided total RNA/mRNAs. Amplified fragment will be ligated in TA vector or in vector of your choice. Two clones will be sequenced for the validity of clones. A project report with detail analysis and 10 ug of final product will be supplied.

cDNA library construction

We offer cDNA library construction service at very competitive price. For details please contact us.

 

 


Related Services

Cell Based Assays
Cell Proliferation Assays
Cell Viability
Cell Adhesion
Cell Migration
Calcium influx
Cytotoxic T-Lymphocyte Assay

 
Protein Services
Protein Isolation/Extraction
Protein Purification
Protein Quantitation
Enzyme Assays
Protein Modifications
PAGE
Western Blot

Custom Polyclonal Antibodies
Antigen Design
Peptide Synthesis
Polyclonal Antibodies
Affinity Purification

Custom Peptide Synthesis:
Design & Synthesis
Up-to 99.9% purity
mg to kg quantities
Mass, HPLC profiles